PCold-assisted expression of a thermostable xylanase from Bacillus amyloliquefaciens: cloning, expression and characterization.

ABSTRACT: The biotechnological application of bacterial xylanases requires a high thermostability, a catalytically active state for a broad pH range. The Bacillus amyloliquefaciens (MTCC 1270) xynA gene was amplified and cloned into the pCold vector and was expressed in Escherichia coli to evaluate the expressed proteins' thermostability. The pCold, compared to other similar vectors, has unique properties-including pH and temperature tolerance due to the presence of the cspA promoter. The recombinant xynA-pCold (rxynApC) showed the expression of xynA gene with a molecular weight of ~ 27 kDa, confirmed on SDS-PAGE. The rxynApC exhibits optimal activity at 70 °C and pH 8.0. The residual activity of the recombinant enzyme was 90% at pH 8.0. The th

SUBMITTER: Patel DK 

PROVIDER: S-EPMC9411286 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature