Ginsenoside Rg5 allosterically interacts with P2RY₁₂ and ameliorates deep venous thrombosis by counteracting neutrophil NETosis and inflammatory response.

ABSTRACT:

Background

Deep venous thrombosis (DVT) highly occurs in patients with severe COVID-19 and probably accounted for their high mortality. DVT formation is a time-dependent inflammatory process in which NETosis plays an important role. However, whether ginsenoside Rg5 from species of Panax genus could alleviate DVT and its underlying mechanism has not been elucidated.

Methods

The interaction between Rg5 and P2RY₁₂ was studied by molecular docking, molecular dynamics, surface plasmon resonance (SPR), and molecular biology assays. The preventive effect of Rg5 on DVT was evaluated in inferior vena cava stasis-induced mice, and immunocytochemistry, Western blot, and calcium flux assay were performed in neutrophils from bone marrow to explore the mechanism of Rg5 i

SUBMITTER: Chen Z 

PROVIDER: S-EPMC9411522 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature