Dataset Information

Bergamot Polyphenol Extract Reduces Hepatocyte Neutral Fat by Increasing Beta-Oxidation.

ABSTRACT:

Background

Bergamot polyphenolic fraction (PF) extract exerts a beneficial against liver steatosis. However, the fundamental processes underlying this beneficial effect of bergamot PF remain elusive. In this work, we examined the effect of bergamot PF extract on 2D and 3D hepatocyte cultures.

Material and methods

We evaluated the effect of bergamot PF in 2D and 3D cultures from rat, human hepatoma cells, and human primary hepatocytes.

Results

In 2D cell culture, we demonstrated that incubation with bergamot PF decreases intracellular lipid content and is associated with an increase in expression levels of ß-oxidation genes (Acox1, Pparα, and Ucp2) and lipophagy (Atg7). Moreover, we confirm this effect on 3D spheroids and organoids.

Conclusion

Incubation with bergamot PF reduces intracellular lipid neutral fat potentially by increasing intracellular pathways related to beta-oxidation.

SUBMITTER: Mirarchi A

PROVIDER: S-EPMC9412422 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature

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Publications

Bergamot Polyphenol Extract Reduces Hepatocyte Neutral Fat by Increasing Beta-Oxidation.

Mirarchi Angela A Mare Rosario R Musolino Vincenzo V Nucera Saverio S Mollace Vincenzo V Pujia Arturo A Montalcini Tiziana T Romeo Stefano S Maurotti Samantha S

Nutrients 20220820 16

<h4>Background</h4>Bergamot polyphenolic fraction (PF) extract exerts a beneficial against liver steatosis. However, the fundamental processes underlying this beneficial effect of bergamot PF remain elusive. In this work, we examined the effect of bergamot PF extract on 2D and 3D hepatocyte cultures.<h4>Material and methods</h4>We evaluated the effect of bergamot PF in 2D and 3D cultures from rat, human hepatoma cells, and human primary hepatocytes.<h4>Results</h4>In 2D cell culture, we demonstr ...[more]

PMID: 36014938

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Dataset Information

Bergamot Polyphenol Extract Reduces Hepatocyte Neutral Fat by Increasing Beta-Oxidation.

Background

Material and methods

Results

Conclusion

Publications

Bergamot Polyphenol Extract Reduces Hepatocyte Neutral Fat by Increasing Beta-Oxidation.

Similar Datasets

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