Molecular Identification of Parasitic Protozoa Sarcocystis in Water Samples.

ABSTRACT: Sarcocystis parasites are among the most common parasitic protozoa in farm animals. So far, the diversity of these parasites has been mainly studied in animal carcasses by morphological or molecular methods. Research on parasitic protozoa in environmental samples is scarce due to the lack of an appropriate methodology and low concentrations of parasites. For these reasons, there is a paucity of validated methods for Sarcocystis identification from environmental samples. Therefore, the present study aims to investigate various molecular methods for Sarcocystis parasite identification in water samples. In the present study, the sample volume, sporocysts isolation, and various conventional PCR were evaluated, and species-specific primers for the identification of differen

SUBMITTER: Strazdaite-Zieliene Z 

PROVIDER: S-EPMC9412564 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature