Cluster Size of Amylopectin and Nanosized Amylopectin Fragments Characterized by Pyrene Excimer Formation.

ABSTRACT: Amylopectin from waxy corn and the three nanosized amylopectin fragments (NAFs)-NAF(56), NAF(20), and NAF(8)-from waxy corn starch with a hydrodynamic diameter of 227, 56, 20, and 8 nm, respectively, were randomly labeled with 1-pyrenebutyric acid. The efficiency of these pyrene-labeled amylopectin-based polysaccharides (Py-AbPS) for pyrene excimer formation (PEF) upon diffusive encounter between an excited and a ground-state pyrene increased with increasing concentration of unlabeled NAF(56) in Py-AbPS dispersions in DMSO. Fluorescence decay analysis of the Py-AbPS dispersions in DMSO prepared with increasing [NAF(56)] yielded the maximum number (N_blob^exp) of anhydroglucose units (AGUs) separating two pyrene-labeled AGUs while still allowi

SUBMITTER: Kim D 

PROVIDER: S-EPMC9412863 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature