Multicolor Super-Resolution Microscopy of Protein Corona on Single Nanoparticles.

ABSTRACT: Nanoparticles represent a promising class of material for nanomedicine and molecular biosensing. The formation of a protein corona due to nonspecific particle-protein interactions is a determining factor for the biological fate of nanoparticles in vivo and strongly impacts the performance of nanoparticles when used as biosensors. Nonspecific interactions are usually highly heterogeneous, yet little is known about the heterogeneity of the protein corona that may lead to inter- and intraparticle differences in composition and protein distribution. Here, we present a super-resolution microscopic approach to study the protein corona on single silica nanoparticles and subsequent cellular interactions using multicolor stimulated emission depletion (STED) microscopy. We demonstrate that STED reso

SUBMITTER: Wang Y 

PROVIDER: S-EPMC9412947 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature