A Pseudovirus Nanoparticle-Based Trivalent Rotavirus Vaccine Candidate Elicits High and Cross P Type Immune Response.

ABSTRACT: Rotavirus infection continues to cause significant morbidity and mortality globally. In this study, we further developed the S₆₀-VP8* pseudovirus nanoparticles (PVNPs) displaying the glycan receptor binding VP8* domains of rotavirus spike proteins as a parenteral vaccine candidate. First, we established a scalable method for the large production of tag-free S₆₀-VP8* PVNPs representing four rotavirus P types, P[8], P[4], P[6], and P[11]. The approach consists of two major steps: selective precipitation of the S-VP8* proteins from bacterial lysates using ammonium sulfate, followed by anion exchange chromatography to further purify the target proteins to a high purity. The purified soluble proteins self-assembled into S₆₀-VP8* PVNPs. Importantly, after intramu

SUBMITTER: Xia M 

PROVIDER: S-EPMC9413348 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature