MRNA-Loaded Lipid Nanoparticles Targeting Dendritic Cells for Cancer Immunotherapy.

ABSTRACT: Dendritic cells (DCs) are attractive antigen-presenting cells to be targeted for vaccinations. However, the systemic delivery of mRNA to DCs is hampered by technical challenges. We recently reported that it is possible to regulate the size of RNA-loaded lipid nanoparticles (LNPs) to over 200 nm with the addition of salt during their formation when a microfluidic device is used and that larger LNPs delivered RNA more efficiently and in greater numbers to splenic DCs compared to the smaller counterparts. In this study, we report on the in vivo optimization of mRNA-loaded LNPs for use in vaccines. The screening included a wide range of methods for controlling particle size in addition to the selection of an appropriate lipid type and its composition. The results showed a clear correlation bet

SUBMITTER: Sasaki K 

PROVIDER: S-EPMC9413374 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature