Molecular architecture of nucleosome remodeling and deacetylase sub-complexes by integrative structure determination.

ABSTRACT: The nucleosome remodeling and deacetylase (NuRD) complex is a chromatin-modifying assembly that regulates gene expression and DNA damage repair. Despite its importance, limited structural information describing the complete NuRD complex is available and a detailed understanding of its mechanism is therefore lacking. Drawing on information from SEC-MALLS, DIA-MS, XLMS, negative-stain EM, X-ray crystallography, NMR spectroscopy, secondary structure predictions, and homology models, we applied Bayesian integrative structure determination to investigate the molecular architecture of three NuRD sub-complexes: MTA1-HDAC1-RBBP4, MTA1^N -HDAC1-MBD3^GATAD2CC , and MTA1-HDAC1-RBBP4-MBD3-GATAD2A [nucleosome deacetylase (NuDe)]. The integrative structures were corroborated by exami

SUBMITTER: Arvindekar S 

PROVIDER: S-EPMC9413472 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature