Toxicity of Orthodontic Brackets Examined by Single Cell Tracking.

ABSTRACT: Subtle toxic effects may be masked in traditional assays that average or summate the response of thousands of cells. We overcome this by using the recent method of single cell tracking in time-lapse recordings. This follows the fate and behavior of individual cells and their progeny and provides unambiguous results for multiple simultaneous biological responses. Further, single cell tracking permits correlation between progeny relationships and cell behavior that is not otherwise possible, including disruption by toxins and toxicants of similarity between paired sister cells. Notably, single cell tracking seems not to have been previously used to study biomaterials toxicity. The culture medium was pre-conditioned by 79 days incubation with orthodontic brackets from seven separate commercia

SUBMITTER: Wishney M 

PROVIDER: S-EPMC9413677 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature