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Anti-Inflammatory Constituents of Antrodia camphorata on RAW 264.7 Cells Induced by Polyinosinic-Polycytidylic Acid.

ABSTRACT: Antrodia camphorata is an endemic mushroom in Taiwan. This study was designed to screen anti-inflammatory compounds from the methanolic extract of the mycelium of A. camphorata on nitric oxide (NO) production in RAW 264.7 cells induced by polyinosinic-polycytidylic acid (poly I:C), a synthetic analog of double-stranded RNA (dsRNA) known to be present in viral infection. A combination of bioactivity-guided isolation with an NMR-based identification led to the isolation of 4-acetylantroquinonol B (1), along with seven compounds. The structure of new compounds (4 and 5) was elucidated by spectroscopic experiments, including MS, IR, and NMR analysis. The anti-inflammatory activity of all isolated compounds was assessed at non-cytotoxic concentrations. 4-Acetylantroquinonol B (1) was the most potent compound against poly I:C-induced NO production in RAW 264.7 cells with an IC50 value of 0.57 ± 0.06 μM.

SUBMITTER: Tu PC 

PROVIDER: S-EPMC9414214 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature

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Anti-Inflammatory Constituents of <i>Antrodia camphorata</i> on RAW 264.7 Cells Induced by Polyinosinic-Polycytidylic Acid.

Tu Ping-Chen PC   Jiang Wen-Ping WP   Lin Ming-Kuem MK   Huang Guan-Jhong GJ   Li Yi-Jen YJ   Kuo Yueh-Hsiung YH  

Molecules (Basel, Switzerland) 20220820 16

<i>Antrodia camphorata</i> is an endemic mushroom in Taiwan. This study was designed to screen anti-inflammatory compounds from the methanolic extract of the mycelium of <i>A. camphorata</i> on nitric oxide (NO) production in RAW 264.7 cells induced by polyinosinic-polycytidylic acid (poly I:C), a synthetic analog of double-stranded RNA (dsRNA) known to be present in viral infection. A combination of bioactivity-guided isolation with an NMR-based identification led to the isolation of 4-acetylan  ...[more]

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