The CpxRA signal transduction system of Escherichia coli: growth-related autoactivation and control of unanticipated target operons.

ABSTRACT: In Escherichia coli, the CpxRA two-component signal transduction system senses and responds to aggregated and misfolded proteins in the bacterial envelope. We show that CpxR-P (the phosphorylated form of the cognate response regulator) activates cpxRA expression in conjunction with RpoS, suggesting an involvement of the Cpx system in stationary-phase survival. Engagement of the CpxRA system in functions beyond protein management is indicated by several putative targets identified after a genomic screening for the CpxR-P recognition consensus sequence. Direct negative control of the newly identified targets motABcheAW (specifying motility and chemotaxis) and tsr (encoding the serine chemoreceptor) by CpxR-P was shown by electrophoretic mobility shift analysis and Northern hybridization. The

SUBMITTER: De Wulf P 

PROVIDER: S-EPMC94143 | biostudies-literature | 1999 Nov

REPOSITORIES: biostudies-literature