Lentinan enhances the antitumor effects of Delta-like 1 via neutrophils.

ABSTRACT:

Background

Selective activation of Delta-like 1 (DLL1)-Notch signaling is a new approach to activate CD8⁺ T cell and suppress tumor growth, while the efficacy remains modest. Lentinan (LNT) is a clinically used immunomodulation agent. Thus, we hypothesized that LNT could improve the efficacy of DLL1.

Methods

The effects of LNT combined with DLL1 on tumor growth were evaluated by growth curve and tumor weight in EO771 breast and LAP0297 lung tumor models. The impacts on immune cells and gene expression in tumor tissues were determined by flow cytometry, qPCR. Neutrophil depletion was used to investigate the mechanism of the combination therapy on tumor growth. The data sets were compared using unpaired student's t-test or ordinary one-way ANOVA.

Results

 LNT

SUBMITTER: Xu H 

PROVIDER: S-EPMC9414423 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature