Isotope Ratio Outlier Analysis (IROA) for HPLC-TOFMS-Based Metabolomics of Human Urine.

ABSTRACT: Metabolic fingerprinting by mass spectrometry aims at the comprehensive, semiquantitative analysis of metabolites. Isotope dilution, if successfully implemented, may provide a more reliable, relative quantification. Therefore, the ¹³C labeled yeast extract of the IROA TruQuant kit was added as an internal standard (IS) to human urine samples measured in full-scan mode on a high-performance liquid chromatography-time-of-flight mass spectrometer (HPLC-TOFMS) system. The isotope ratio approach enabled the analysis of 112 metabolites. The correlation with reference data did not improve significantly using ¹²C/¹³C ratios compared to absolute ¹²C peak areas. Moreover, using an intricate ¹³C-labeled standard increased the complexity of the ma

SUBMITTER: Fadil F 

PROVIDER: S-EPMC9414531 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature