Outer membrane lipoprotein e (P4) of Haemophilus influenzae is a novel phosphomonoesterase.

ABSTRACT: Haemophilus influenzae exists as a commensal of the upper respiratory tract of humans but also causes infections of contiguous structures. We describe the identification, localization, purification, and characterization of a novel, surface-localized phosphomonoesterase from a nontypeable H. influenzae strain, R2866. Sequences obtained from two CNBr-derived fragments of this protein matched lipoprotein e (P4) within the H. influenzae sequence database. Escherichia coli DH5alpha transformed with plasmids containing the H. influenzae hel gene, which encodes lipoprotein e (P4), produced high levels of a membrane-associated phosphomonoesterase. The isolated approximately 28-kDa enzyme was tartrate resistant and displayed narrow substrate specificity with the highest activity for arylphosphates,

SUBMITTER: Reilly TJ 

PROVIDER: S-EPMC94146 | biostudies-literature | 1999 Nov

REPOSITORIES: biostudies-literature