Dataset Information

Proliferation and Apoptosis of Cat (Felis catus) Male Germ Cells during Breeding and Non-Breeding Seasons.

ABSTRACT: The domestic cat (Felis catus) is a seasonal-breeding species whose reproductive period starts when the day length increases. Since the existing information on cat spermatogenesis is limited and somewhat contradictory, in the present study, germ cell proliferation and apoptosis in feral adult tomcats orchiectomized during reproductive (reproductive group, RG; February-July) and non-reproductive (non-reproductive group, NRG; November and December) seasons were compared. Cross-sections taken from the middle third of the left testis were chemically fixed and embedded in paraffin wax. Histological sections were processed for the immunohistochemical detection of proliferating germ cells (PCNA) and for the identification of apoptotic cells (TUNEL method). The percentage of PCNA-positive spermatogonia was higher in the RG than in the NRG. On the contrary, germ cell apoptosis was higher in the NRG than in the RG. Our results confirm that cat spermatogenesis is modulated on a seasonal basis and suggests that spermatogenesis control involves changes in germ cell proliferation and apoptosis according to a common paradigm of seasonally breeding species.

SUBMITTER: Valentini L

PROVIDER: S-EPMC9414637 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Proliferation and Apoptosis of Cat (<i>Felis catus</i>) Male Germ Cells during Breeding and Non-Breeding Seasons.

Valentini Luisa L Zupa Rosa R Pousis Chrysovalentinos C Cuko Rezart R Corriero Aldo A

Veterinary sciences 20220820 8

The domestic cat (<i>Felis catus</i>) is a seasonal-breeding species whose reproductive period starts when the day length increases. Since the existing information on cat spermatogenesis is limited and somewhat contradictory, in the present study, germ cell proliferation and apoptosis in feral adult tomcats orchiectomized during reproductive (reproductive group, RG; February-July) and non-reproductive (non-reproductive group, NRG; November and December) seasons were compared. Cross-sections take ...[more]

PMID: 36006362

Similar Datasets

Project description:Domestic cats are popular companion animals, however not all live in human homes and many cats live within shelters or as free-roaming, unowned- feral or stray cats. Cats can transition between these subpopulations, but the influence of this connectivity on overall population dynamics, and the effectiveness of management interventions, remain poorly understood. We developed a UK-focused multistate Matrix Population Model (MPM), combining multiple life history parameters into an integrated model of cat demography and population dynamics. The model characterises cats according to their age, subpopulation and reproductive status, resulting in a 28-state model. We account for density-dependence, seasonality and uncertainty in our modelled projections. Through simulations, we examine the model by testing the effect of different female owned-cat neutering scenarios over a 10-year projection timespan. We also use the model to identify the vital rates to which total population growth is most sensitive. The current model framework demonstrates that increased prevalence of neutering within the owned cat subpopulation influences the population dynamics of all subpopulations. Further simulations find that neutering owned cats younger is sufficient to reduce overall population growth rate, regardless of the overall neutering prevalence. Population growth rate is most influenced by owned cat survival and fecundity. Owned cats, which made up the majority of our modelled population, have the most influence on overall population dynamics, followed by stray, feral and then shelter cats. Due to the importance of owned-cat parameters within the current model framework, we find cat population dynamics are most sensitive to shifts in owned cat husbandry. Our results provide a first evaluation of the demography of the domestic cat population in the UK and provide the first structured population model of its kind, thus contributing to a wider understanding of the importance of modelling connectivity between subpopulations. Through example scenarios we highlight the importance of studying domestic cat populations in their entirety to better understand factors influencing their dynamics and to guide management planning. The model provides a theoretical framework for further development, tailoring to specific geographies and experimental investigation of management interventions.

Dataset Information

Proliferation and Apoptosis of Cat (Felis catus) Male Germ Cells during Breeding and Non-Breeding Seasons.

Publications

Proliferation and Apoptosis of Cat (<i>Felis catus</i>) Male Germ Cells during Breeding and Non-Breeding Seasons.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets