Membrane Chromatography-Based Downstream Processing for Cell-Culture Produced Influenza Vaccines.

ABSTRACT: New influenza strains are constantly emerging, causing seasonal epidemics and raising concerns to the risk of a new global pandemic. Since vaccination is an effective method to prevent the spread of the disease and reduce its severity, the development of robust bioprocesses for producing pandemic influenza vaccines is exceptionally important. Herein, a membrane chromatography-based downstream processing platform with a demonstrated industrial application potential was established. Cell culture-derived influenza virus H1N1/A/PR/8/34 was harvested from benchtop bioreactor cultures. For the clarification of the cell culture broth, a depth filtration was selected as an alternative to centrifugation. After inactivation, an anion exchange chromatography membrane was used for viral capture and fu

SUBMITTER: Yang Z 

PROVIDER: S-EPMC9414887 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature