A Micro-Scale Analytical Method for Determining Glycogen Turnover by NMR and FTMS.

ABSTRACT: Glycogen is a readily deployed intracellular energy storage macromolecule composed of branched chains of glucose anchored to the protein glycogenin. Although glycogen primarily occurs in the liver and muscle, it is found in most tissues, and its metabolism has been shown to be important in cancers and immune cells. Robust analysis of glycogen turnover requires stable isotope tracing plus a reliable means of quantifying total and labeled glycogen derived from precursors such as ¹³C₆-glucose. Current methods for analyzing glycogen are time- and sample-consuming, at best semi-quantitative, and unable to measure stable isotope enrichment. Here we describe a microscale method for quantifying both intact and acid-hydrolyzed glycogen by ultra-high-resolution Fourier transfor

SUBMITTER: Scott TL 

PROVIDER: S-EPMC9415681 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature