Rapid and Accurate Detection of <i>Gnomoniopsis smithogilvyi</i> the Causal Agent of Chestnut Rot, through an Internally Controlled Multiplex PCR Assay.

ABSTRACT: The fungus Gnomoniopsis smithogilvyi is a significant threat to the production of sweet chestnut (Castanea sativa) nuts in Australia and worldwide. The pathogen causes nut rot, which leads to substantial production losses. Early and accurate diagnosis of the disease is essential to delineate and implement control strategies. A specific and sensitive multiplex PCR was developed based on the amplification of three barcode sequences of G. smithogilvyi. The assay reliability was enhanced by including the amplification of a host gene as an internal control. Primers were thoroughly evaluated in silico before assessing them in vitro. Primer annealing temperature and concentration were optimised to enhance the assay sensitivity and specificity. The assay detection limit ranged

SUBMITTER: Silva-Campos M 

PROVIDER: S-EPMC9415963 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature