TLC-Densitometry for Determination of Omeprazole in Simple and Combined Pharmaceutical Preparations.

ABSTRACT: TLC combined with densitometry was used and chromatographic conditions developed to separate omeprazole and diclofenac sodium from their potential impurities. The development of the TLC-densitometry method is based on the elaboration of new chromatographic conditions allowing for the simultaneous determination of omeprazole and diclofenac sodium in a pharmaceutical preparation. Identification and quantification of omeprazole in simple and combined (with diclofenac) pharmaceutical preparations was performed on silica gel 60F₂₅₄ using one mobile phase: chloroform-methanol-ammonia (36:4:0.60, v/v). Diclofenac sodium was determined in the presence of omeprazole after 2D separation on silica gel using two mobile phases of the first phase of chloroform-methanol-ammonia (

SUBMITTER: Parys W 

PROVIDER: S-EPMC9416117 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature