Grapevine Badnavirus 1: Detection, Genetic Diversity, and Distribution in Croatia.

ABSTRACT: Grapevine badnavirus 1 (GBV-1) was recently discovered in grapevine using high throughput sequencing. In order to carry out large-scale testing that will allow for better insights into virus distribution, conventional and real-time PCR assays were developed using sequences both from previously known, and four newly characterized isolates. Throughout the growing season and dormancy, GBV-1 can be detected by real-time PCR using available tissue, with the possibility of false-negative results early in vegetation growth. GBV-1 real-time PCR analysis of 4302 grapevine samples from the Croatian continental and coastal wine-growing regions revealed 576 (~13.4%) positive vines. In the continental wine-growing region, virus incidence was confirmed in only two collection plantations, whereas in the

SUBMITTER: Jagunic M 

PROVIDER: S-EPMC9416389 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature