Structural Fingerprinting of siRNA Therapeutics by Solution NMR Spectroscopy.

ABSTRACT: Nucleic acids are an increasingly popular platform for the development of biotherapeutics to treat a wide variety of illnesses, including diseases where traditional drug development efforts have failed. To date, there are 14 short oligonucleotide therapeutics and 2 messenger RNA (mRNA) vaccines approved by the U.S. Food and Drug Administration (FDA), which demonstrates the potential of nucleic acids as a platform for the development of safe and effective medicines and vaccines. Despite the increasing popularity of nucleic acid-based drugs, there has been a paucity of high-resolution structural techniques applied to rigorously characterize these molecules during drug development. Here, we present application of nuclear magnetic resonance (NMR) methods to structurally "fingerprint" short oli

SUBMITTER: Becette OB 

PROVIDER: S-EPMC9416564 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature