Monitoring decellularization via absorbance spectroscopy during the derivation of extracellular matrix scaffolds.

ABSTRACT: Extracellular matrix (ECM) is a complex structure composed of bioactive molecules representative of the local tissue microenvironment. Decellularized ECM biomaterials harness these biomolecules for regenerative medicine applications. One potential therapeutic application is the use of vocal fold (VF) specific ECM to restore the VFs after injury. ECM scaffolds are derived through a process of decellularization, which aims to remove unwanted immunogenic biomolecules (e.g. DNA) while preserving the composition of the ECM. The effectiveness of the decellularization is typically assessed at the end by quantifying ECM attributes such as final dsDNA content. However, batch-to-batch variability in ECM manufacturing remains a significant challenge for the standardization, cost-effectiveness, and sc

SUBMITTER: Mora-Navarro C 

PROVIDER: S-EPMC9416610 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature