Insights into the internal structures of nanogels using a versatile asymmetric-flow field-flow fractionation method.

ABSTRACT: Poly(N-isopropylacrylamide) (pNIPAM) nanogels are a highly researched type of colloidal material. In this work, we establish a versatile asymmetric-flow field-flow fractionation (AF4) method that can provide high resolution particle sizing and also structural information on nanogel samples from 65-310 nm in hydrodynamic diameter and so different chemical compositions. To achieve this online multi-angle light scattering and dynamic light scattering detectors were used to provide measurement of the radius of gyration (R _g) and hydrodynamic radius (R _h) respectively. Two different eluents and a range of cross-flows were evaluated in order to provide effective fractionation and high recovery for the different nanogel samples. We found that using 0.1 M

SUBMITTER: Niezabitowska E 

PROVIDER: S-EPMC9416902 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature