MtDNA variability determines spontaneous joint aging damage in a conplastic mouse model.

ABSTRACT: Mitochondria and mtDNA variations contribute to specific aspects of the aging process. Here, we aimed to investigate the influence of mtDNA variation on joint damage in a model of aging using conplastic mice. A conplastic (BL/6^NZB) mouse strain was developed with the C57BL/6JOlaHsd nuclear genome and NZB/OlaHsd mtDNA, for comparison with the original C57BL/6JOlaHsd strain (BL/6^C57). Conplastic (BL/6^NZB) and BL/6^C57 mice were sacrificed at 25, 75, and 90 weeks of age. Hind knee joints were processed for histological analysis and joint pathology graded using the Mankin scoring system. By immunohistochemistry, cartilage expression of markers of autophagy (LC3, Beclin-1, and P62) and markers of senescence (MMP13, beta-Galactosidase, and p16) and prol

SUBMITTER: Scotece M 

PROVIDER: S-EPMC9417242 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature