Developing a rational approach to designing recombinant proteins for peptide-directed nanoparticle synthesis.

ABSTRACT: The controlled formation of nanoparticles with optimum characteristics and functional aspects has proven successful via peptide-mediated nanoparticle synthesis. However, the effects of the peptide sequence and binding motif on surface features and physicochemical properties of nanoparticles are not well-understood. In this study, we investigate in a comparative manner how a specific peptide known as Pd4 and its two known variants may form nanoparticles both in an isolated state and when attached to a green fluorescent protein (GFPuv). More importantly, we introduce a novel computational approach to predict the trend of the size and activity of the peptide-directed nanoparticles by estimating the binding affinity of the peptide to a single ion. We used molecular dynamics (MD) simulat

SUBMITTER: Polasa A 

PROVIDER: S-EPMC9417332 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature