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Neurite guidance and neuro-caging on steps and grooves in 2.5 dimensions.

ABSTRACT: Directed guidance of neurites is a pre-requisite for tailor-made designs of interfaces between cells and semiconducting components. Grayscale lithography, reactive ion etching, and ultraviolet nanoimprint lithography are potent semiconductor industry-compatible techniques for a cost- and time-effective fabrication of modulated surfaces. In this work, neurite outgrowth of murine cerebellar neurons on 2.5D pathways produced with these methods is studied. Structures of micron-sized steps and grooves serve as cell culture platforms. The effects of contact guidance through topography and chemical guidance through selective poly-d-lysine coating on these platforms are analyzed. As a consequence, the herein presented fabrication approach can be utilized to cultivate and to study low-density neuronal networks in 2.5D configuration with a high degree of order.

SUBMITTER: Fendler C

PROVIDER: S-EPMC9417336 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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Neurite guidance and neuro-caging on steps and grooves in 2.5 dimensions.

Fendler Cornelius C Harberts Jann J Rafeldt Lars L Loers Gabriele G Zierold Robert R Blick Robert H RH

Nanoscale advances 20200714 11

Directed guidance of neurites is a pre-requisite for tailor-made designs of interfaces between cells and semiconducting components. Grayscale lithography, reactive ion etching, and ultraviolet nanoimprint lithography are potent semiconductor industry-compatible techniques for a cost- and time-effective fabrication of modulated surfaces. In this work, neurite outgrowth of murine cerebellar neurons on 2.5D pathways produced with these methods is studied. Structures of micron-sized steps and groove ...[more]

PMID: 36132017

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