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Moderate molecular recognitions on ZnO m-plane and their selective capture/release of bio-related phosphoric acids.

ABSTRACT: Herein, we explore the hidden molecular recognition abilities of ZnO nanowires uniformly grown on the inner surface of an open tubular fused silica capillary via liquid chromatography. Chromatographic evaluation revealed that ZnO nanowires showed a stronger intermolecular interaction with phenylphosphoric acid than any other monosubstituted benzene. Furthermore, ZnO nanowires specifically recognized the phosphate groups present in nucleotides even in the aqueous mobile phase, and the intermolecular interaction increased with the number of phosphate groups. This discrimination of phosphate groups in nucleotides was unique to the rich (101̄0) m-plane of ZnO nanowires with a moderate hydrophilicity and negative charge. The discrimination could be evidenced by the changes in the infrared bands of the phosphate groups on nucleotides on ZnO nanowires. Finally, as an application of the molecular recognition, nucleotides were separated by the number of phosphate groups, utilizing optimized gradient elution on ZnO nanowire column. Thus, the present results elucidate the unique and versatile molecular selectivity of well-known ZnO nanostructures for the capture and separation of biomolecules.

SUBMITTER: Kanao E

PROVIDER: S-EPMC9417451 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature

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Moderate molecular recognitions on ZnO <i>m</i>-plane and their selective capture/release of bio-related phosphoric acids.

Kanao Eisuke E Nakano Katsuya K Kamei Ryoma R Hosomi Takuro T Ishihama Yasushi Y Adachi Jun J Kubo Takuya T Otsuka Koji K Yanagida Takeshi T

Nanoscale advances 20220217 6

Herein, we explore the hidden molecular recognition abilities of ZnO nanowires uniformly grown on the inner surface of an open tubular fused silica capillary <i>via</i> liquid chromatography. Chromatographic evaluation revealed that ZnO nanowires showed a stronger intermolecular interaction with phenylphosphoric acid than any other monosubstituted benzene. Furthermore, ZnO nanowires specifically recognized the phosphate groups present in nucleotides even in the aqueous mobile phase, and the inte ...[more]

PMID: 36134362

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Project description:The existing methods for exosome isolation, such as ultracentrifugation, size exclusion, and affinity separation, suffer from some limitations. Herein, we aimed to develop temperature-modulated exosome-capturing materials using thermoresponsive polymers and peptides with affinity for exosomes. Poly(2-hydroxyethyl methacrylate-co-propargyl acrylate)-b-poly(N-isopropylacrylamide) (P(HEMA-co-PgA)-b-PNIPAAm) was grafted on silica beads via a two-step process of activator regenerated by electron transfer atom transfer radical polymerization. Peptides with affinity for exosomes were conjugated to the propargyl group of the bottom P(HEMA-co-PgA) segment of the copolymer via a click reaction. The prepared copolymer-grafted beads were characterized by elemental analysis, X-ray photoelectron spectroscopy, scanning electron microscopy, transmission electron microscopy, gel permeation chromatography, and the turbidity of the polymer solution. Results indicated that the copolymer and peptide were successfully modified on the silica beads. Exosomes from SK-BR-3 cells, a human breast cancer cell line, were selectively captured on the prepared beads at 37 °C, as the upper PNIPAAm segment shrank and the affinity between the peptide and exosome was enhanced. Upon lowering the temperature to 4 °C, the captured exosomes were released from the copolymer brush because of the extension of the PNIPAAm segment that reduced the affinity between peptides and exosomes. These findings demonstrated that the prepared copolymer brush-grafted silica beads can capture and release targeted exosomes via temperature modulation. Taken together, the developed copolymer brush-grafted silica beads would be useful for the separation of exosomes using simple procedures such as temperature modulation. Graphical abstract Image 1 Highlights • Functional beads were developed using thermoresponsive polymer and affinity peptide.• Exosome selectively captured on beads through affinity between the peptide and HER2.• Captured exosome was released from the beads via extension of the polymer.• Temperature-modulated capture of exosome was achieved via temperature change.

Dataset Information

Moderate molecular recognitions on ZnO m-plane and their selective capture/release of bio-related phosphoric acids.

Publications

Moderate molecular recognitions on ZnO <i>m</i>-plane and their selective capture/release of bio-related phosphoric acids.

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