Enhancing FRET biosensing beyond 10 nm with photon avalanche nanoparticles.

ABSTRACT: Förster Resonance Energy Transfer (FRET) between donor (D) and acceptor (A) molecules is a phenomenon commonly exploited to study or visualize biological interactions at the molecular level. However, commonly used organic D and A molecules often suffer from photobleaching and spectral bleed-through, and their spectral properties hinder quantitative analysis. Lanthanide-doped upconverting nanoparticles (UCNPs) as alternative D species offer significant improvements in terms of photostability, spectral purity and background-free luminescence detection, but they bring new challenges related to multiple donor ions existing in a single large size UCNP and the need for nanoparticle biofunctionalization. Considering the relatively short Förster distance (typically below 5-7 nm), it becomes a non-

SUBMITTER: Bednarkiewicz A 

PROVIDER: S-EPMC9417941 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature