Enhanced avidin binding to lipid bilayers using PDP-PE lipids with PEG-biotin linkers.

ABSTRACT: Two of the most important aspects of lipid bilayers that have increased their popularity in the field of nanotechnology and biosensors are their fluid nature, which is highly beneficial in ensuring the spatial organization of attached molecules, and the relative ease in which they can be manipulated to change the surface chemistry. Here we have used two different types of functionalized lipids to study the interaction of avidin, which is a common approach to attach further ligands for study. We have tested the commonly used Biotinyl-Cap-PE lipids at different molar percentages and reveal that avidin is not evenly distributed, but forms what looks like clusters even at low percentage occupancy which hampers the level of avidin that can be associated with the surface. We have then successful

SUBMITTER: Birchenough HL 

PROVIDER: S-EPMC9417969 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature