Reproducible measurable residual disease detection by multiparametric flow cytometry in acute myeloid leukemia.

ABSTRACT: Measurable residual disease (MRD) detected by multiparametric flow cytometry (MFC) is associated with unfavorable outcome in patients with AML. A simple, broadly applicable eight-color panel was implemented and analyzed utilizing a hierarchical gating strategy with fixed gates to develop a clear-cut LAIP-based DfN approach. In total, 32 subpopulations with aberrant phenotypes with/without expression of markers of immaturity were monitored in 246 AML patients after completion of induction chemotherapy. Reference values were established utilizing 90 leukemia-free controls. Overall, 73% of patients achieved a response by cytomorphology. In responders, the overall survival was shorter for MRD^pos patients (HR 3.8, p = 0.006). Overall survival of MRD^neg non-responders was c

SUBMITTER: Rohnert MA 

PROVIDER: S-EPMC9417981 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature