The oxidoreductase CLIC4 is required to maintain mitochondrial function and resistance to exogenous oxidants in breast cancer cells.

ABSTRACT: The chloride intracellular channel-4 (CLIC4) is one of the six highly conserved proteins in the CLIC family that share high structural homology with GST-omega in the GST superfamily. While CLIC4 is a multifunctional protein that resides in multiple cellular compartments, the discovery of its enzymatic glutaredoxin-like activity in vitro suggested that it could function as an antioxidant. Here, we found that deleting CLIC4 from murine 6DT1 breast tumor cells using CRISPR enhanced the accumulation of reactive oxygen species (ROS) and sensitized cells to apoptosis in response to H₂O₂ as a ROS-inducing agent. In intact cells, H₂O₂ increased the expression of both CLIC4 mRNA and protein. In addition, increased superoxide production in 6DT1 cells lacki

SUBMITTER: Al Khamici H 

PROVIDER: S-EPMC9418444 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature