Optimal centrifugal isolating of liposome-protein complexes from human plasma.

ABSTRACT: In the past few years, characterization of the protein corona (PC) that forms around liposomal systems has gained increasing interest for the development of novel therapeutic and diagnostic technologies. At the crossroads of fast-moving research fields, the interdisciplinarity of protein corona investigations poses challenges for experimental design and reporting. Isolation of liposome-protein complexes from biological fluids has been identified as a fundamental step of the entire workflow of PC characterization but exact specifications for conditions to optimize pelleting remain elusive. In the present work, key factors affecting precipitation of liposome-protein complexes by centrifugation, including time of centrifugation, total sample volume, lipid : protein ratio and contamination fro

SUBMITTER: Digiacomo L 

PROVIDER: S-EPMC9418580 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature