Real-time surface-enhanced Raman scattering-based live cell monitoring of the changes in mitochondrial membrane potential.

ABSTRACT: Obtaining molecular information on cells in real time has been a critical challenge in studying the interaction between molecules of interest and intracellular components. Fluorescence-based methods have long served as excellent tools to study such important interactions. In this paper, we introduce a Raman scattering-based method as a promising platform to achieve the real-time monitoring of subtle molecular changes occurring within cells. We found that the Raman scattering-based method enabled monitoring changes in the mitochondrial membrane potential at the single-cell level in rheumatoid arthritis synovial fibroblasts induced by tumor necrosis factor-alpha (TNF-α) protein, various chemicals (MgCl₂, FCCP, and sodium pyruvate), and a non-chemical stimulus (i.e., light).

SUBMITTER: Lee JH 

PROVIDER: S-EPMC9418680 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature