Magnetic nanoparticle mediated-gene delivery for simpler and more effective transformation of Pichia pastoris.

ABSTRACT: The introduction of exogenous DNA into a cell can be used to produce large quantities of protein. Here, we describe a novel gene delivery method for Pichia pastoris based on recombinant DNA delivery using magnetic nanoparticles (MNPs) under magnetic forces. For this purpose, a linear plasmid (pGKB-GFP) containing the Green Fluorescent Protein (GFP) gene is loaded on polyethyleneimine-coated iron oxide (Fe₃O₄@PEI) MNPs at doses that are non-toxic to the yeast cells. The pGKB-GFP loaded MNPs combined with enhancer PEI (Fe₃O₄@PEI + pGKB-GFP + PEI) are directly transferred to non-competent cells. An effective GFP expression was observed by the selection of antibiotic-resistant yeast cells and heterologous gene integration into the P. pastor

SUBMITTER: Yildiz S 

PROVIDER: S-EPMC9418747 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature