Investigation of Bacteria from Spoiled Bottled Salad Dressing Leading to Gas Explosion.

ABSTRACT:

Background

In 2020, an incident involving spoiled salad dressing from a commercial source occurred. Upon opening the bottle, the contents exploded from gas that seemed to have fermented inside the bottle. For safety concerns, we sought to investigate the bacteria from the salad dressing in order to notify the company that made the product and relevant authorities.

Methods

Anaerobic and carbon dioxide culture methods were used. To determine species of colonies, MALDI-TOF-MS and 16S rRNA whole sequencing were performed.

Results

There were no colonies grown in anaerobic condition; however, we obtained three colonies from the carbon dioxide atmosphere. We determined the first colony as Alkalihalobacillus clausii (Bacillus clausii), the second as Bacillus<

SUBMITTER: Obata F 

PROVIDER: S-EPMC9419222 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature