Raltitrexed regulates proliferation and apoptosis of HGC-27 cells by upregulating RSK4.

ABSTRACT:

Background

Raltitrexed is a specific inhibitor of thymidylate synthase and a potential chemotherapeutic agent for the treatment of advanced gastric cancer. In this study, we investigated the effect of raltitrexed on the proliferation of HGC-27 human gastric cancer cells and its potential underlying molecular mechanism(s).

Methods

RT-qPCR and western blotting were used to quantify RSK4 levels. Colony formation and flow cytometry assays were used to assess HGC-27 cell proliferation, cell cycle progression, mitochondrial membrane potential, and apoptosis. The expression of cell cycle and apoptosis markers were determined by western blotting.

Results

Our results demonstrate that raltitrexed upregulated RSK4 mRNA and protein levels in HGC-27 cells. Moreover, raltitrexed si

SUBMITTER: Hu C 

PROVIDER: S-EPMC9420250 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature