Secretion of collagenases by Saccharomyces cerevisiae for collagen degradation.

ABSTRACT:

Background

The production and processing of animal-based products generates many collagen-rich by-products, which have received attention both for exploitation to increase their added value and to reduce their negative environmental impact. The collagen-rich by-products can be hydrolyzed by collagenases for further utilization. Therefore, collagenases are of benefit for efficient collagen materials processing. An alternative and safe way to produce secreted collagenases is needed.

Results

Two collagenases from Hathewaya histolytica, ColG and ColH, were successfully secreted by the yeast Saccharomyces cerevisiae. Compared with the native signal peptide of collagenase, the α-factor leader is more efficient in guiding collagenase secretion. Collagenase secretion was significant

SUBMITTER: Xiao H 

PROVIDER: S-EPMC9420286 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature