Improved fluorescent signal in expansion microscopy using fluorescent Fab fragment secondary antibodies.

ABSTRACT: Expansion microscopy (ExM) is a microscopic imaging approach that can achieve super-resolution visualization of fluorescently labeled biological samples using conventional fluorescence microscopy. The method is based on embedding of a fluorescently labeled biological sample in a hydrogel matrix followed by the physical expansion of the specimen, which is then viewed using a conventional fluorescent microscope. Variations of the method can be used to visualize endogenously expressed fluorescent proteins, such as GFP, fluorescently tagged antibodies, nucleic acids, or other fluorescently tagged molecules. A significant challenge of the method is that the physical expansion of the specimen produces a concommitant reduction in fluorescence intensity, which can make imaging difficult. We descri

SUBMITTER: Sherry DM 

PROVIDER: S-EPMC9420380 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature