Generating transcriptional regulatory networks from time-ordered stem cell differentiation RNA sequencing data.

ABSTRACT: We describe steps to 1) identify ascending and descending monotonic key genes from time-ordered stem cell differentiation expression data, 2) construct time-ordered transcriptional regulatory networks, and 3) infer the involvement of transcription factors along the differentiation process. For complete details on the use and execution of this protocol, please refer to Wong et al. (2020).

SUBMITTER: Tsai YS 

PROVIDER: S-EPMC9420390 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature