Recovery of reduced thiol groups by superoxide-mediated denitrosation of nitrosothiols.

ABSTRACT: Nitrosation of critical thiols has been elaborated as reversible posttranslational modification with regulatory function in multiple disorders. Reversibility of S-nitrosation is generally associated with enzyme-mediated one-electron reductions, catalyzed by the thioredoxin system, or by nitrosoglutathione reductase. In the present study, we confirm previous evidence for a non-enzymatic de-nitrosation of nitrosoglutathione (GSNO) by superoxide. The interaction leads to the release of nitric oxide that subsequently interacts with a second molecule of superoxide (O₂^•-) to form peroxynitrite. Despite the formation of peroxynitrite, approximately 40-70% of GSNO yielded reduced glutathione (GSH), depending on the applied analytical assay. The concept of O₂^•-

SUBMITTER: Schildknecht S 

PROVIDER: S-EPMC9420518 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature