Live functional assays reveal longitudinal maturation of transepithelial transport in kidney organoids.

ABSTRACT: Kidney organoids derived from hPSCs have opened new opportunities to develop kidney models for preclinical studies and immunocompatible kidney tissues for regeneration. Organoids resemble native nephrons that consist of filtration units and tubules, yet little is known about the functional capacity of these organoid structures. Transcriptomic analyses provide insight into maturation and transporter activities that represent kidney functions. However, functional assays in organoids are necessary to demonstrate the activity of these transport proteins in live tissues. The three-dimensional (3D) architecture adds complexity to real-time assays in kidney organoids. Here, we develop a functional assay using live imaging to assess transepithelial transport of rhodamine 123 (Rh123), a fluorescent

SUBMITTER: Rizki-Safitri A 

PROVIDER: S-EPMC9420851 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature