The effects of continuous exposure to low-dose chlorine dioxide gas on the characteristics of induced pluripotent stem cells.

ABSTRACT:

Background

Recently, various regenerative therapies have been developed based on induced pluripotent stem (iPS) cells. However, hygienic control strategies have not been established at the manufacturing facilities. We aimed to evaluate the safety and effects of continuous exposure to low-dose chlorine dioxide (ClO₂) gas on cell fates, and to determine the optimum dose for safe usage of this disinfectant.

Methods

We cultured an iPS cell line in the absence or presence of various doses of ClO₂ gas. We evaluated cell proliferation, cell death, the maintenance of undifferentiated state, and cell senescence.

Results

We found that iPS cell proliferation was not affected by 0.05 or 0.1 ppmv ClO₂ gas in the atmosphere. Although 0.1 ppmv ClO

SUBMITTER: Okawa R 

PROVIDER: S-EPMC9420961 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature