Novel electrochemical and electrochemiluminescence dual-modality sensing platform for sensitive determination of antimicrobial peptides based on probe encapsulated liposome and nanochannel array electrode.

ABSTRACT: With the increasing application of antimicrobial peptides (AMPs) to replace antibiotics in medicine, food and agriculture, it is highly desired to develop a fast, reliable, and convenient strategy for sensitive detection of AMPs. Herein, a novel electrochemical (EC) and electrochemiluminescence (ECL) dual-modality sensing platform was developed based on probe encapsulated liposomes and nanochannel array modified electrodes, which enables sensitive determination of nisin in food samples. The bifunctional probe with both EC and ECL signals, tris(2,2-bipyridyl) dichlororuthenium (II) (Ru(bpy)₃ ²⁺), was chosen to be easily encapsulated in liposomes (Ru(bpy)₃ ²⁺@liposome). Based on the unique sterilization mechanism that AMPs can disrupt cell membrane

SUBMITTER: Luo X 

PROVIDER: S-EPMC9421287 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature