Study on the molecular mechanisms of tetrandrine against pulmonary fibrosis based on network pharmacology, molecular docking and experimental verification.

ABSTRACT:

Aims

This study aims to screen the potential targets of tetrandrine (Tet) against pulmonary fibrosis (PF) based on network pharmacological analysis, molecular docking and experimental verification.

Main methods

The network pharmacology methods were employed to predict targets, construct Tet-PF-intersection target-pathway networks, and screen the candidate targets. The molecular docking was performed using AutoDockTools1.5.6. TGF-β1-induced human lung adenocarcinoma A549 cells were used as an in vitro experimental verification model, taking dexamethasone (Dex) as the positive control, to verify the effects of Tet on the mRNA expression of the candidate targets.

Key findings

Six candidate targets were predicted based on network pharmacology and molecular docking,

SUBMITTER: Li J 

PROVIDER: S-EPMC9421403 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature