Meso-seq for in-depth transcriptomics in ultra-low amounts of FACS-purified neuronal nuclei.

ABSTRACT: Profiling of gene expression in sparse populations of genetically defined neurons is essential for dissecting the molecular mechanisms that control the development and plasticity of neural circuits. However, current transcriptomic approaches are ill suited for detailed mechanistic studies in sparse neuronal populations, as they either are technically complex and relatively expensive (e.g., single-cell RNA sequencing [RNA-seq]) or require large amounts of input material (e.g., traditional bulk RNA-seq). Thus, we established Meso-seq, a meso-scale protocol for identifying more than 10,000 robustly expressed genes in as little as 50 FACS-sorted neuronal nuclei. We demonstrate that Meso-seq works well for multiple neuroscience applications, including transcriptomics in antibody-labeled cortica

SUBMITTER: Apelblat D 

PROVIDER: S-EPMC9421536 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature