Clustering of Cryptosporidium species infections among sheep and cattle but not children in remote highland communities of Madagascar.

ABSTRACT:

Background

The aim of this study was to identify local transmission patterns of Cryptosporidium spp. infections among livestock and humans in four extremely rural and remote highland communities in Madagascar.

Methods

In this cross-sectional study, households were randomly sampled throughout a 1-year study period, with one feces sample collected from each child (≤ 5 years old), sheep and cattle. Cryptosporidium spp. were identified using a nested PCR assay targeting the 18S ribosomal RNA gene. All samples positive for Cryptosporidium hominis were further subtyped by sequencing the 60-kDa glycoprotein gene (gp60). Spatial clustering methods were applied to analyze potential transmission patterns.

Results

In total, 252 households participated in the study, and samples f

SUBMITTER: Krumkamp R 

PROVIDER: S-EPMC9422120 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature