Novel flavonoid C-8 hydroxylase from Rhodotorula glutinis: identification, characterization and substrate scope.

ABSTRACT:

Background

The regioselective hydroxylation of phenolic compounds, especially flavonoids, is still a bottleneck of classical organic chemistry that could be solved using enzymes with high activity and specificity. Yeast Rhodotorula glutinis KCh735 in known to catalyze the C-8 hydroxylation of flavones and flavanones. The enzyme F8H (flavonoid C8-hydroxylase) is involved in the reaction, but the specific gene has not yet been identified. In this work, we present identification, heterologous expression and characterization of the first F8H ortho-hydroxylase from yeast.

Results

Differential transcriptome analysis and homology to bacterial monooxygenases, including also a FAD-dependent motif and a GD motif characteristic for flavin-dependent monooxygenases, provided a set of cod

SUBMITTER: Dulak K 

PROVIDER: S-EPMC9422121 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature