Membrane transporters control cerebrospinal fluid formation independently of conventional osmosis to modulate intracranial pressure.

ABSTRACT:

Background

Disturbances in the brain fluid balance can lead to life-threatening elevation in the intracranial pressure (ICP), which represents a vast clinical challenge. Nevertheless, the details underlying the molecular mechanisms governing cerebrospinal fluid (CSF) secretion are largely unresolved, thus preventing targeted and efficient pharmaceutical therapy of cerebral pathologies involving elevated ICP.

Methods

Experimental rats were employed for in vivo determinations of CSF secretion rates, ICP, blood pressure and ex vivo excised choroid plexus for morphological analysis and quantification of expression and activity of various transport proteins. CSF and blood extractions from rats, pigs, and humans were employed for osmolality determinations and a mathematical model

SUBMITTER: Oernbo EK 

PROVIDER: S-EPMC9422132 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature