Genomic characterization of Staphylococcus aureus isolates causing osteoarticular infections in otherwise healthy children.

ABSTRACT:

Background

Pediatric osteoarticular infections are commonly caused by Staphylococcus aureus. The contribution of S. aureus genomic variability to pathogenesis of these infections is poorly described.

Methods

We prospectively enrolled 47 children over 3 1/2 years from whom S. aureus was isolated on culture-12 uninfected with skin colonization, 16 with skin abscesses, 19 with osteoarticular infections (four with septic arthritis, three with acute osteomyelitis, six with acute osteomyelitis and septic arthritis and six with chronic osteomyelitis). Isolates underwent whole genome sequencing, with assessment for 254 virulence genes and any mutations as well as creation of a phylogenetic tree. Finally, isolates were compared for their ability to form static biofilms and compared t

SUBMITTER: Dehority W 

PROVIDER: S-EPMC9423648 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature